antibodies against α sma  (Novus Biologicals)

Journal: ACS Omega

Article Title: The N‑Glycoproteomic Landscape of the Lung in Monocrotaline-Induced Pulmonary Arterial Hypertension

doi: 10.1021/acsomega.6c02324

Figure Lengend Snippet: The flowchart of this study and the validation of MCT-induced PAH rat model. A: The flowchart of the study. B: The typical waveform of pulmonary arterial pressure in MCT-induced PAH rats and control (Ctrl) rats. C–D: The mPAP and RVHI of PAH and control rats. E: H&E staining of lung tissues in PAH rats and controls. F–G. The WT% and WA% of PAH and control rats. H: The results of α-SMA (green), CD68 (purple), and DAPI (blue) staining in PAH lungs at ×400 magnification. The data were expressed as mean ± SD ( n = 8); * p < 0.05 vs Ctrl.

Article Snippet: Formalin-fixed, paraffin-embedded lung sections (4 μm) were deparaffinized in xylene, rehydrated through a graded ethanol series (100% to 50%), and subjected to antigen retrieval in EDTA buffer (pH 9.0) at 95 °C for 20 min. After cooling to room temperature and washing with PBS, sections were blocked with 10% nonfat milk for 30 min and then incubated overnight at 4 °C with primary antibodies against α-SMA (1:800; GB13044–50, Servicebio, China), CD68 (1:200; ab318303, Abcam, UK), VCAM1 (1:200, ET1601-18, Huabio, China), and AMBP (1:1000, ER1803-35, Huabio, China).

Techniques: Biomarker Discovery, Control, Staining

Journal: ACS Omega

Article Title: The N‑Glycoproteomic Landscape of the Lung in Monocrotaline-Induced Pulmonary Arterial Hypertension

doi: 10.1021/acsomega.6c02324

Figure Lengend Snippet: Elevation of VCAM1 and AMBP in lungs of MCT-induced PAH rats. A–B: Proteomic and N-glycoproteomic analyses revealed an increase in total protein level and N423-site glycosylation of VCAM1 in MCT-treated rats. C–D: Total protein level and N233-site glycosylation of AMBP were elevated in MCT-treated rats. E: Representative Western blot images showing protein expression of VCAM1 and AMBP in lung tissues. F–G: Quantitative analysis of VCAM1 and AMBP protein levels. H–I: Immunofluorescence results of VCAM1 and AMBP expression in lung tissues. Representative images show α-SMA (red), VCAM1 (green, H) or AMBP (green, I), DAPI (blue), and the merged channels (Merge) at ×400 magnification. Data are presented as mean ± SD; n = 10 (Ctrl), n = 8 (MCT); * p < 0.05, ** p < 0.01, *** p < 0.001 vs Ctrl.

Article Snippet: Formalin-fixed, paraffin-embedded lung sections (4 μm) were deparaffinized in xylene, rehydrated through a graded ethanol series (100% to 50%), and subjected to antigen retrieval in EDTA buffer (pH 9.0) at 95 °C for 20 min. After cooling to room temperature and washing with PBS, sections were blocked with 10% nonfat milk for 30 min and then incubated overnight at 4 °C with primary antibodies against α-SMA (1:800; GB13044–50, Servicebio, China), CD68 (1:200; ab318303, Abcam, UK), VCAM1 (1:200, ET1601-18, Huabio, China), and AMBP (1:1000, ER1803-35, Huabio, China).

Techniques: Glycoproteomics, Western Blot, Expressing, Immunofluorescence

Journal: Brazilian Journal of Cardiovascular Surgery

Article Title: Optimization of the in vitro Model of Cardiac Fibrosis

doi: 10.21470/1678-9741-2025-0075

Figure Lengend Snippet: The expression of alpha-smooth muscle actin (α-SMA) protein. A) Immunostaining of α-SMA. (B): Quantification of α-SMA intensity expression in positive cells. Data are expressed as mean ± standard deviation (three separate experiments with three replicates in each). *P < 0.05. AA=ascorbic acid; Ang II=angiotensin II; Dex=dextran sulfate.

Article Snippet: Next, the cells were incubated with primary antibodies against α-SMA (mouse, Zytomed Systems) or vimentin (1:400, mouse, Sigma, Germany) 75 minutes at room temperature and overnight at 4°C, respectively.

Techniques: Expressing, Immunostaining, Standard Deviation

Journal: Frontiers in Pharmacology

Article Title: Coenzyme Q10 alleviates silicotic fibrosis in rats through the TGF-β1/Smad pathway

doi: 10.3389/fphar.2026.1821205

Figure Lengend Snippet: Effects of CoQ10 on fibrosis-related proteins in rat lung tissue induced by SiO 2 dust suspension. ( (A) Effect of CoQ10 on HYP content in rat lungs induced by silica dust; (B) Effect of CoQ10 on the expression of EMT-related proteins in rat lungs induced by silica dust; (C) E-cad protein expression level; (D) Vimentin protein expression level; (E) α-SMA protein expression level; (F) Immunohistochemical detection of α-SMA protein expression (10×); (G) α-SMA protein positive expression level; (H) Effect of CoQ10 on collagen protein expression in rat lung tissue induced by silica dust; (I) Collagen-I protein expression level; (J) Collagen-III protein expression level. Note: * indicates P < 0.05 compared with the Control group; ** indicates P < 0.01 compared with the Control group; # indicates P < 0.05 compared with the Silicosis Model group; ## indicates P < 0.01 compared with the Silicosis Model group. All data are presented as mean ± standard deviation (M ± SD), n = 3).

Article Snippet: Free silica dust (SiO 2 , with ≥80% of particles measuring 0.5–5 μm, Sigma-Aldrich, United States); Coenzyme Q10 (Macklin Biochemical Technology Co., Ltd., Shanghai, China); Kits for Interleukin-1β (IL-1β), Tumor Necrosis Factor-α (TNF-α), Superoxide Dismutase (SOD), and Malondialdehyde (MDA) (Nanjing Jiancheng Bioengineering Institute, China); Hematoxylin and Eosin (H&E) staining solution, Mayer’s Hematoxylin, Eosin Y solution, Masson’s Trichrome Stain Kit, Reactive Oxygen Species (ROS) Staining Kit (Wuhan Biqindu Biotechnology Co., Ltd., China); Tween 20, Rapid Wright-Giemsa Stain, Bicinchoninic Acid (BCA) Protein Assay Kit (Beijing Dingguo Changsheng Biotechnology Co., Ltd., China); Antibodies against α-Smooth Muscle Actin (α-SMA), E-cadherin (E-cad), Vimentin, Collagen Type III (Col-III), Smad2, Smad3 (Abclonal Technology Co., Ltd., Wuhan, China); Antibodies against Collagen Type I (Col-I), Smad7 (Wuhan Sanying Biotechnology Co., Ltd., China); Antibody against Transforming Growth Factor Beta 1 (TGF-β1) (Abcam, China); Horseradish Peroxidase (HRP)-conjugated Goat Anti-Rabbit antibody (Abclonal, China).

Techniques: Suspension, Expressing, Immunohistochemical staining, Control, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Role and mechanism of tetrahedral DNA nanostructures in the repair of urethral injury in rats

doi: 10.3892/mmr.2026.13815

Figure Lengend Snippet: (A) Masson's trichrome staining and immunohistochemistry results. Masson's trichrome staining detected changes in collagen fibers in urethral tissues, with collagen fibers appearing blue and muscle fibers, fibrin and red blood cells appearing red. Immunohistochemistry staining showed the cell nuclei in blue and positive protein staining for the fibrosis markers α-SMA, TGF-β1, collagen I, collagen III and Smad3 in brown. Images are presented at a magnification of ×200. (B) Statistical analysis of Masson's trichrome staining and immunohistochemistry results. Staining results were analyzed with Image-Pro Plus software(version 6.0; Media Cybernetics, Inc.), followed by bar chart construction using GraphPad Prism. *P<0.05, **P<0.01 and ***P<0.001. (C) Western blot analysis results. Western blot analysis was used to detect the protein expression of fibrosis markers in urethral tissue. (D) Statistical analysis of western blotting results. Band densities were analyzed using Image-Pro Plus software(version 6.0; Media Cybernetics, Inc.), and bar charts were drawn with GraphPad Prism. *P<0.05, **P<0.01 and ***P<0.001. α-SMA, α-smooth muscle actin; TDN, tetrahedral DNA nanostructure.

Article Snippet: The cells were incubated with primary antibodies against α-SMA (1:100; cat. no. AF1032; Affinity Biosciences) and CD90 (1:100; cat. no. DF4804; Affinity Biosciences) overnight at 4°C.

Techniques: Staining, Immunohistochemistry, Software, Western Blot, Expressing

Journal: Molecular Medicine Reports

Article Title: Role and mechanism of tetrahedral DNA nanostructures in the repair of urethral injury in rats

doi: 10.3892/mmr.2026.13815

Figure Lengend Snippet: (A) Fibroblast isolation and cultivation. Representative phase-contrast images of primary rat urethral fibroblasts following enzymatic digestion are shown. The two images represent different microscopic fields from the same primary culture, illustrating progressive cell adhesion and the typical spindle-shaped, adherent morphology with elongated cell bodies and prominent cytoplasmic extensions. (B) H&E staining. Cell nuclei were stained darkly, with the cytoplasm stained lightly and clear cell boundaries visible, with cells displaying the normal morphological characteristics of fibroblasts after H&E staining. (C) Immunofluorescence staining identification of fibroblasts. Immunofluorescence staining for α-SMA (green) and CD90 (red) was performed to verify fibroblast purity. Nuclei were counterstained with DAPI (blue) and imaged at a magnification of ×200 using a Nikon TS2-S-SM fluorescence microscope. Cells exhibited strong CD90 expression, α-SMA staining was negative, whereas CD90 staining was clearly positive, confirming fibroblast identity and excluding myofibroblast contamination. (D) Immunohistochemical detection of vimentin. Vimentin expression in fibroblasts was detected by immunohistochemistry. The nuclei were stained blue, and vimentin-positive cytoplasm appeared brownish-yellow. Images were obtained at a magnification of ×200 using a digital slide scanner. (E) Immunohistochemical detection of vimentin. Quantitative analysis among three replicates was performed using Image-Pro Plus, and statistical evaluation with GraphPad Prism 9.0 showed no significant differences among groups (P>0.05), confirming reproducibility. (F) Cell culture under different treatments. Representative phase-contrast images (magnification, ×100) showed fibroblasts in the control, TGF-β and TGF-β + TDN groups, all displaying sufficient adherence and morphology. (G) Quantitative analysis of vimentin expression under different treatments. Vimentin expression levels in fibroblasts from the control, TGF-β and TGF-β + TDN groups were quantified based on immunohistochemical staining using Image-Pro Plus. Statistical analysis was performed using GraphPad Prism 9.0 (*P<0.05, **P<0.01). (H) Immunohistochemical detection of vimentin under different treatments. Representative immunohistochemical images showed vimentin expression in fibroblasts from the Control, TGF-β and TGF-β + TDN groups. α-SMA, α-smooth muscle actin; TDN, tetrahedral DNA nanostructure.

Article Snippet: The cells were incubated with primary antibodies against α-SMA (1:100; cat. no. AF1032; Affinity Biosciences) and CD90 (1:100; cat. no. DF4804; Affinity Biosciences) overnight at 4°C.

Techniques: Isolation, Staining, Immunofluorescence, Fluorescence, Microscopy, Expressing, Immunohistochemical staining, Immunohistochemistry, Cell Culture, Control